Smart microscopy represents a paradigm shift in biological imaging, moving from passive observation tools to active collaborators in scientific inquiry. Enabled by advances in automation, computational power, and artificial intelligence, these systems are now capable of adaptive decision-making and real-time experimental control. Here, we introduce a theoretical framework that reconceptualizes smart microscopy as a partner in scientific investigation. Central to our framework is the concept of the 'epistemic-empirical divide' in cellular investigation-the gap between what is observable (empirical domain) and what must be understood (epistemic domain). We propose six core design principles: epistemic-empirical awareness, hierarchical context integration, an evolution from detection to perception, adaptive measurement frameworks, narrative synthesis capabilities, and cross-contextual reasoning. Together, these principles guide a multi-agent architecture designed to align empirical observation with the goals of scientific understanding. Our framework provides a roadmap for building microscopy systems that go beyond automation to actively support hypothesis generation, insight discovery, and theory development, redefining the role of scientific instruments in the process of knowledge creation.
In response to the call for agent-based solutions that leverage the ever-increasing capabilities of the deep models' ecosystem, we introduce Hive -- a comprehensive solution for selecting appropriate models and subsequently planning a set of atomic actions to satisfy the end-users' instructions. Hive operates over sets of models and, upon receiving natural language instructions (i.e. user queries), schedules and executes explainable plans of atomic actions. These actions can involve one or more of the available models to achieve the overall task, while respecting end-users specific constraints. Notably, Hive handles tasks that involve multi-modal inputs and outputs, enabling it to handle complex, real-world queries. Our system is capable of planning complex chains of actions while guaranteeing explainability, using an LLM-based formal logic backbone empowered by PDDL operations. We introduce the MuSE benchmark in order to offer a comprehensive evaluation of the multi-modal capabilities of agent systems. Our findings show that our framework redefines the state-of-the-art for task selection, outperforming other competing systems that plan operations across multiple models while offering transparency guarantees while fully adhering to user constraints.
Large Language Models (LLMs) often perpetuate biases in pronoun usage, leading to misrepresentation or exclusion of queer individuals. This paper addresses the specific problem of biased pronoun usage in LLM outputs, particularly the inappropriate use of traditionally gendered pronouns ("he," "she") when inclusive language is needed to accurately represent all identities. We introduce a collaborative agent pipeline designed to mitigate these biases by analyzing and optimizing pronoun usage for inclusivity. Our multi-agent framework includes specialized agents for both bias detection and correction. Experimental evaluations using the Tango dataset-a benchmark focused on gender pronoun usage-demonstrate that our approach significantly improves inclusive pronoun classification, achieving a 32.6 percentage point increase over GPT-4o in correctly disagreeing with inappropriate traditionally gendered pronouns $(\chi^2 = 38.57, p < 0.0001)$. These results accentuate the potential of agent-driven frameworks in enhancing fairness and inclusivity in AI-generated content, demonstrating their efficacy in reducing biases and promoting socially responsible AI.
PRefLexOR (Preference-based Recursive Language Modeling for Exploratory Optimization of Reasoning) combines preference optimization with concepts from Reinforcement Learning to enable models to self-teach through iterative reasoning improvements. We propose a recursive learning approach that engages the model in multi-step reasoning, revisiting, and refining intermediate steps before producing a final output in training and inference phases. Through multiple training stages, the model first learns to align its reasoning with accurate decision paths by optimizing the log odds between preferred and non-preferred responses. During this process, PRefLexOR builds a dynamic knowledge graph by generating questions from random text chunks and retrieval-augmentation to contextualize relevant details from the entire training corpus. In the second stage, preference optimization enhances model performance by using rejection sampling to fine-tune reasoning quality by continually producing in-situ training data while masking the reasoning steps. Recursive optimization within a thinking token framework introduces iterative feedback loops, where the model refines reasoning, achieving deeper coherence, consistency, and adaptability. Implemented in small language models with only 3 billion parameters, we should that even tiny models can iteratively teach themselves to reason with greater depth and reflectivity. Our implementation is straightforward and can be incorporated into any existing pretrained LLM. We focus our examples on applications in biological materials science and demonstrate the method in a variety of case studies that range from in-domain to cross-domain applications. Using reasoning strategies that include thinking and reflection modalities we build a multi-agent recursive self-improving inference approach to successively improve responses via repeated sampling in inference time.
Large Language Model Multi-Agent Systems (LLM-MAS) have achieved great progress in solving complex tasks. It performs communication among agents within the system to collaboratively solve tasks, under the premise of shared information. However, when agents' collaborations are leveraged to perform multi-person tasks, a new challenge arises due to information asymmetry, since each agent can only access the information of its human user. Previous MAS struggle to complete tasks under this condition. To address this, we propose a new MAS paradigm termed iAgents, which denotes Informative Multi-Agent Systems. In iAgents, the human social network is mirrored in the agent network, where agents proactively exchange human information necessary for task resolution, thereby overcoming information asymmetry. iAgents employs a novel agent reasoning mechanism, InfoNav, to navigate agents' communication toward effective information exchange. Together with InfoNav, iAgents organizes human information in a mixed memory to provide agents with accurate and comprehensive information for exchange. Additionally, we introduce InformativeBench, the first benchmark tailored for evaluating LLM agents' task-solving ability under information asymmetry. Experimental results show that iAgents can collaborate within a social network of 140 individuals and 588 relationships, autonomously communicate over 30 turns, and retrieve information from nearly 70,000 messages to complete tasks within 3 minutes.
Despite extensive pre-training in moral alignment to prevent generating harmful information, large language models (LLMs) remain vulnerable to jailbreak attacks. In this paper, we propose AutoDefense, a multi-agent defense framework that filters harmful responses from LLMs. With the response-filtering mechanism, our framework is robust against different jailbreak attack prompts, and can be used to defend different victim models. AutoDefense assigns different roles to LLM agents and employs them to complete the defense task collaboratively. The division in tasks enhances the overall instruction-following of LLMs and enables the integration of other defense components as tools. With AutoDefense, small open-source LMs can serve as agents and defend larger models against jailbreak attacks. Our experiments show that AutoDefense can effectively defense against different jailbreak attacks, while maintaining the performance at normal user request. For example, we reduce the attack success rate on GPT-3.5 from 55.74% to 7.95% using LLaMA-2-13b with a 3-agent system. Our code and data are publicly available at https://github.com/XHMY/AutoDefense.
With the rapid advancement of Large Language Models (LLMs), significant progress has been made in multi-agent applications. However, the complexities in coordinating agents' cooperation and LLMs' erratic performance pose notable challenges in developing robust and efficient multi-agent applications. To tackle these challenges, we propose AgentScope, a developer-centric multi-agent platform with message exchange as its core communication mechanism. The abundant syntactic tools, built-in agents and service functions, user-friendly interfaces for application demonstration and utility monitor, zero-code programming workstation, and automatic prompt tuning mechanism significantly lower the barriers to both development and deployment. Towards robust and flexible multi-agent application, AgentScope provides both built-in and customizable fault tolerance mechanisms. At the same time, it is also armed with system-level support for managing and utilizing multi-modal data, tools, and external knowledge. Additionally, we design an actor-based distribution framework, enabling easy conversion between local and distributed deployments and automatic parallel optimization without extra effort. With these features, AgentScope empowers developers to build applications that fully realize the potential of intelligent agents. We have released AgentScope at https://github.com/modelscope/agentscope, and hope AgentScope invites wider participation and innovation in this fast-moving field.
The increasing volume of commercially available conversational agents (CAs) on the market has resulted in users being burdened with learning and adopting multiple agents to accomplish their tasks. Though prior work has explored supporting a multitude of domains within the design of a single agent, the interaction experience suffers due to the large action space of desired capabilities. To address these problems, we introduce a new task BBAI: Black-Box Agent Integration, focusing on combining the capabilities of multiple black-box CAs at scale. We explore two techniques: question agent pairing and question response pairing aimed at resolving this task. Leveraging these techniques, we design One For All (OFA), a scalable system that provides a unified interface to interact with multiple CAs. Additionally, we introduce MARS: Multi-Agent Response Selection, a new encoder model for question response pairing that jointly encodes user question and agent response pairs. We demonstrate that OFA is able to automatically and accurately integrate an ensemble of commercially available CAs spanning disparate domains. Specifically, using the MARS encoder we achieve the highest accuracy on our BBAI task, outperforming strong baselines.
Nowadays, IoT devices have an enlarging scope of activities spanning from sensing, computing to acting and even more, learning, reasoning and planning. As the number of IoT applications increases, these objects are becoming more and more ubiquitous. Therefore, they need to adapt their functionality in response to the uncertainties of their environment to achieve their goals. In Human-centered IoT, objects and devices have direct interactions with human beings and have access to online contextual information. Self-adaptation of such applications is a crucial subject that needs to be addressed in a way that respects human goals and human values. Hence, IoT applications must be equipped with self-adaptation techniques to manage their run-time uncertainties locally or in cooperation with each other. This paper presents SMASH: a multi-agent approach for self-adaptation of IoT applications in human-centered environments. In this paper, we have considered the Smart Home as the case study of smart environments. SMASH agents are provided with a 4-layer architecture based on the BDI agent model that integrates human values with goal-reasoning, planning, and acting. It also takes advantage of a semantic-enabled platform called Home'In to address interoperability issues among non-identical agents and devices with heterogeneous protocols and data formats. This approach is compared with the literature and is validated by developing a scenario as the proof of concept. The timely responses of SMASH agents show the feasibility of the proposed approach in human-centered environments.